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bcip(r)/nbt-blue liquid substrate system for membranes

BCIP(R)/NBT-BLUE LIQUID SUBSTRATE SYSTEM FOR MEMBRANES

Catalog No.
C005B-100607
Manufacturer No.
B3804-100ML
Manufacturer Name
Sigma-Aldrich
Quantity
100
Unit of Measure
ML

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Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan

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General description

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-bromo4-chloro-3-indolyl phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end-product that is blue to purple and can be observed visually. BCIP®/NBT-blue liquid substrate system for membranes is a specifically formulated liquid enzyme substrate that is stable and optimized for blotting applications offers maximum convenience, time savings, and consistency.

Application

BCIP®/NBT-Blue Liquid Substrate System for Membranes has been used in colorimetric detection of alkaline phosphatase (ALP) activity such as western blot analysis.

Caution

Stable at least one year at 2-8 Â°C. This substrate is light-sensitive.

Other Notes

Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Quality Level: 100. form: liquid. storage temp.: 2-8°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.
UPC:
12161702
Condition:
New
HazmatClass:
No
MPN:
B3804-100ML

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