BCIP/NBT Stock Solution, 50X

5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on Western, Northern, Southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive.|Recommended for use with Crystal Mounting Medium (sc-24943).Preparation of staining solution:•Add 200 µl of BCIP/NBT stock solution to the following room temperature buffer: 10 ml 0.1 M Tris Hydrochloride, pH 9.5, 0.1 M Sodium Chloride, and 0.05 M Magnesium Chloride. (do not include Magnesium Chloride in the detecBCIP/NBT Stock Solution, 50Xis a substrate for alkaline phosphatase (AP) based detection systems including western blotting.

Specifications and Purity: 50 X

MDL Number: MFCD00466954

Molecular Formula: NBT: C40H30Cl2N10O6, BCIP: C8H6NO4BrClP x C7H9N

EC Number: 229-506-1 (BCIP), 206-067-4 (NBT)}

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