BCH869 HUMAN H3K27M GLIOMA CELL LINES (C005B-315638)

General description

"Glioma, a type of tumor that occurs in the brain and spinal cord and originates in the supportive glial cells surrounding nerve cells, is one of the most common types of primary brain tumors. Low-grade gliomas are the predominant glioma diagnosis among children, with very good prognosis and extremely rare malignant progression. Conversely, high-grade gliomas (HGG) are aggressive cancers and considered one of the most lethal tumors affecting the pediatric age group.1 Diffuse midline gliomas (DMG) with histone H3 lysine27-to-methionine mutations (H3K27M glioma) represent a highly aggressive subtype of glioma, which predominantly arise in children and young adults. The overall prognosis of H3K27M glioma is poor, displaying median survival rates of approximately 9 to 11 months.2

BCH245 cell line is a patient-derived model of H3K27M glioma.3 BCH245 cells grow as neurospheres in suspension culture and have been characterized via expression of epidermal growth factor receptor (EGFR). BCH245 cells have demonstrated utility in unraveling the mechanisms of glioma tumorigenesis and physiology,4 representing a clinically relevant model for this especially serious type of glioma.

Source
BCH245 was derived from diffuse midline glioma in Pons tissue of a male pediatric patient.3

References
1. Sci Rep 2020, 10(1): 8368
2. Front Oncol 2020, 9:1436.
3. Science 2018, 360(6386): 331-335.
4. Nat Commun 2019, 10(1): 1262."

Application

• Each vial contains > 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Human Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of human origin and negative for inter-species contamination from mouse, rat, chinese hamster, Golden Syrian hamster, and Non-human Primate (NHP) as assessed by a Contamination Clear panel by Charles River Animal Diagnostic Services
• Cells are negative for mycoplasma contamination.

Glioma, a type of tumor that occurs in the brain and spinal cord and originates in the supportive glial cells surrounding nerve cells, is one of the most common types of primary brain tumors. Low-grade gliomas are the predominant glioma diagnosis among children, with very good prognosis and extremely rare malignant progression. Conversely, high-grade gliomas (HGG) are aggressive cancers and considered one of the most lethal tumors affecting the pediatric age group.1 Diffuse midline gliomas (DMG) with histone H3 lysine27-to-methionine mutations (H3K27M glioma) represent a highly aggressive subtype of glioma, which predominantly arise in children and young adults. The overall prognosis of H3K27M glioma is poor, displaying median survival rates of approximately 9 to 11 months.2

BCH245 cell line is a patient-derived model of H3K27M glioma.3 BCH245 cells grow as neurospheres in suspension culture and have been characterized via expression of epidermal growth factor receptor (EGFR). BCH245 cells have demonstrated utility in unraveling the mechanisms of glioma tumorigenesis and physiology,4 representing a clinically relevant model for this especially serious type of glioma.

Source
BCH245 was derived from diffuse midline glioma in Pons tissue of a male pediatric patient.3

References
1. Sci Rep 2020, 10(1): 8368
2. Front Oncol 2020, 9:1436.
3. Science 2018, 360(6386): 331-335.
4. Nat Commun 2019, 10(1): 1262.

Features and Benefits

BCH245 cells have demonstrated utility in unraveling the mechanisms of glioma tumorigenesis and physiology,4 representing a clinically relevant model for this especially serious type of glioma.

Storage and Stability

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.