BAFILOMYCIN A1 (C005B-086332)

General description

Chemical structure: macrolide

Application

Bafilomycin A1 has been used:

• as an autophagy inhibitor to study its effects on hepatic steatosis in human hepatocytes
• as a vacuolar-type H⁺ -ATPase inhibitor to study its effects on the cell viability, percentage of PI-positive cells, and glucose-stimulated insulin secretion (GSIS) in mice INS-1 cells
• as a vacuolar-type H⁺ -ATPase inhibitor to study its effects on tau degradation in human SH-SY5Y cells

Biochem/physiol Actions

A specific inhibitor of vacuolar type H⁺-ATPase (V-ATPase) in animal cells, plant cells and microorganisms.

Bafilomycin A1 inhibits autophagosome-lysosome fusion and autolysosome acidification, the steps involved in the autophagic process that is required for maintaining functional autophagic flux and cellular homeostasis.

Molecular Weight: 622.83. Empirical Formula: C₃₅H₅₈O₉. Quality Level: 300. Assay: ≥. 90% (HPLC). form: film. antibiotic activity spectrum: fungi. Mode of action: DNA synthesis | interferes, enzyme | inhibits. storage temp.: −. 20°C. SMILES string: CO[C@H]1\C: C\C: C(C)\C[C@H](C)[C@H](O)[C@H](C)\C: C(C)\C: C(OC)C(: O)OC1[C@@H](C)[C@@H](O)[C@H](C)[C@@]2(O)C[C@@H](O)[C@H](C)[C@H](O2)C(C)C. InChI: 1S/C35H58O9/c1-19(2)32-24(7)27(36)18-35(40,44-32)26(9)31(38)25(8)33-28(41-10)14-12-13-20(3)15-22(5)30(37)23(6)16-21(4)17-29(42-11)34(39)43-33/h12-14,16-17,19,22-28,30-33,36-38,40H,15,18H2,1-11H3/b14-12+,20-13+,21-16+,29-17-/t22-,23+,24-,25-,26-,27+,28-,30-,31+,32+,33+,35+/m0/s1. InChI key: XDHNQDDQEHDUTM-JQWOJBOSSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.