ATTO 488 MALEIMIDE BIOREAGENT FOR FLUORESCENCE >= 90% (COUPLING TO THIOLS) (C005B-226860)

General description

Atto 488 Maleimide is a hydrophilic fluorescent label with high molecular absorption (90.000) and quantum yield (0.80). The fluorescence activity is excited at a maximum of 501nm, and the maximum emission is 523nm. Atto 488 Maleimide is optimized for argon laser excitation and characterized by high photostability. Hydrolysis of maleimides to a mixture of isomeric nonreactive maleamic acids can compete significantly with thiol modification, particularly above pH 8. Post coupling to a substrate, the label carries a net electrical charge of -1.

Application

Atto 488 Maleimide is used for labelling amines, which usually requires a higher pH than the reaction of maleimides with thiols. It is a well-suited fluorescent label for thiol groups.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

product line: BioReagent. Quality Level: 100. Assay: ≥. 90% (HPLC), ≥. 90% (degree of coupling ). manufacturer/tradename: ATTO-TEC GmbH. λ: in methanol: water (1:1) (with 0.1% perchloric acid). UV absorption: λ. : 501-506 . nm A_max. suitability: suitable for fluorescence. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).