ATTO 425-NHS ESTER BIOREAGENT FOR FLUORESCENCE (C005B-086921)

General description

Atto 425 is a member of the commercially available coumarin derivatives.

Application

Atto 425 NHS ester is used in the development of bifunctional fluorescent, inorganic micro-partices (Zeolite L crystals) designed for targeting or imaging mutliple components . Reacts with amino groups or amine modified oliognucleotides to form a stable amine bond. Utilized as an essential component of molecular beacons, which were designed for use in real-time PCR assays to allow for rapid and accurate identification of gram-negative bacteria . When conjugated with benzylguanine, the fluorescence of Atto 425-NHS is quenched but becomes fluorescent upon labeling of the SNAP-tag fusion protein, making this conjugate an ideal candidate for experiments in living cells . Used as one of the donor probes in three-color FRET experiments designed to investigate the kinetics of RNA-protein complex formation .

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation.

Other Notes

Study of stability during one- and two-photon excitation in fluorescence correlation spectroscopy, FCS; employed in triple-color coincidence analysis.

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Molecular Weight: 498.53. Empirical Formula: C₂₆H₃₀N₂O₈. product line: BioReagent. Quality Level: 100. Assay: ≥. 90% (HPLC), ≥. 90% (degree of coupling). manufacturer/tradename: ATTO-TEC GmbH. solubility: DMF: soluble, DMSO: soluble, H₂O: soluble. λ: in ethanol (with 0.1% trifluoroacetic acid). UV absorption: λ. : 422-428 . nm A_max. suitability: suitable for fluorescence. storage temp.: −. 20°C. SMILES string: CCOC(: O)C1: Cc2cc3C(C)CC(C)(C)N(CCCC(: O)ON4C(: O)CCC4: O)c3cc2OC1: O. InChI: 1S/C26H30N2O8/c1-5-34-24(32)18-12-16-11-17-15(2)14-26(3,4)27(19(17)13-20(16)35-25(18)33)10-6-7-23(31)36-28-21(29)8-9-22(28)30/h11-13,15H,5-10,14H2,1-4H3. InChI key: PUEQEMDTFPYCDY-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).