ANTI-VITAMIN D RECEPTOR (AB- 1PC X 100UG (C005B-200305)

General description

Purified rat monoclonal antibody generated by immunizing rats with the specified immunogen and fusing splenocytes with mouse myeloma p3 NS 1/Ag4-1 (NS1) mouse myeloma cells. Recognizes both the occupied and unoccupied forms of vitamin D receptor.

Recognizes both occupied and unoccupied vitamin D receptor in breast carcinomas expressing the vitamin D receptor protein.

This Anti-Vitamin D Receptor (Ab-1) Rat mAb (9A7γE10.4) is validated for use in Frozen Sections, Gel Shift, WB, IP, Paraffin Sections for the detection of Vitamin D Receptor (Ab-1).

Immunogen

purified, chick intestine vitamin D receptor

Application

Frozen Sections (1:50-1:100)

Gel Shift (see comments)

Immunoblotting (1:200-1:400)

Immunoprecipitation (10 µl/mg protein lysate)

Paraffin Sections (1:50-1:100, sample pre-treatment required, see comments)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Analysis Note

Positive Control
Breast carcinoma tissue (see comments)

Other Notes

Brown, A.J. 1999. Nephrol. Dial. Transplant.14, 11.
Fleet, J.C. 1999. Nutr. Rev.57 60.
Evans, S.R., et al. 1998. Clin. Cancer Res.4, 1591.
Friedrich, M., et al. 1998. J. Histochem. Cytochem.46, 1335.
Issa, L.L., et al. 1998. Inflamm. Res.47, 451.
Kivineva, M., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 121.
Nangia, A.K., et al. 1998. J. Steroid Biochem. Mol. Biol.66, 241.

Does not cross-react with estrogen and glucocorticoid receptors. About 50% of breast tumors express vitamin D receptor protein. For gel shift assays, order antibody at 1 mg/ml; contact Customer Service for additional information. For paraffin sections pre-treatment in 2N HCl for 20 min at 37°C prior to staining. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rat. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: 9A7γ. E10.4, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: chicken, hamster, mouse, porcine, rat, amphibian, human. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG1. shipped in: wet ice. storage temp.: 2-8°C. target post-translational modification: unmodified. Gene Information: human ... VDR(7421). Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.