ANTI-SNF2BETA/BRG1 ANTIBODY

General description

The SNF2 family of proteins are ATP dependant chromatin remodeling enzymes. The gene encoding this protein is also refered to as SMARCA4.

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI-SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI-SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor).

Specificity

This antibody recognizes the Near N-Terminus of SNF2B

Immunogen

GST-tagged recombinant protein corresponding to the Near N-Terminus of human SNF2β.

Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected BRG1 in HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Transcription Factors

Use Anti-SNF2β/BRG1 Antibody (rabbit polyclonal antibody) validated in IP, WB to detect SNF2β/BRG1 also known as Probable global transcription activator SNF2L4, ATP-dependent helicase SMARCA4, SNF2-beta, BRG-1.

Quality

Evaluated by Western Blotting in HELA NE cell lysate.
Western Blotting Analysis: A 1:4,000 dilution of this antibody detected SNF2B/BRG1 in 10 µg of HELA NE cell lysate.

Target description

200 kDa

Physical form

Rabbit polyclonal in buffer containing with 0.05% sodium azide with 30% glycerol.

Unpurified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.