ANTI-NOX4 CLONE 10A8.1 (MOUSE MONOCLONAL)

General description

NADPH oxidase 4 (UniProt Q9NPH5; also known as Kidney oxidase-1, Kidney superoxide-producing NADPH oxidase, KOX-1, Renal NAD(P)H-oxidase) is encoded by the NOX4 (also known as RENOX) gene (Gene ID 50507) in human. NOX4 belongs to the family of NADPH oxidases that modulate redox-dependent cellular signalling pathways via intracellular reactive oxygen species (ROS) generation. Seven family members have been identified in mammals (NOX1–5 and DUOX1/2) with member-specific cellular distribution, intracellular location, mechanisms of action and types of ROS produced. NOX4 produces predominantly H2O2, and is widely expressed in many cell types and adult tissues, including kidney, liver, heart and aorta. NOX4 plays an important role in homocysteine (Hcy) metabolism, which in turns is a determining factor that impacts on defence against environmental toxins. Nox-knocout in mice results in significantly decreased total plasma Hcy levels, causing markedly increased susceptibility to acetaminophen- (APAP-, paracetamol-) induced hepatoxicity as a result of significantly decreased cysteine production and hepatic GSH stores. Human NOX4 is a six-transmembrane (a.a. 17-37, 63-83, 104-124, 155-175, 189-209, 425-445) protein, having both its N- and C-terminal ends at the cytoplasmic side (a.a. 1-16 & 446-578).

Specificity

Immunogen construct region is present in human NOX4 spliced isoform 1, 2 (Nox4A), and 8, missing in isoform 5 (Nox4C), 7, and 9, partially truncated in isoform 3 (Nox4E), 4 (Nox4D), and 6 (Nox4B) reported by UniProt (Q9NPH5).

Immunogen

Epitope: Third extracellular loop.

GST-tagged recombinant protein corresponding to a fragment from the third extracellular loop of human NOX4.

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NOX4 in human kidney, pancreas, and testis tissue sections.

Research Category
Apoptosis & Cancer

Research Sub Category
Toxicology & Drug Resistance

This Anti-NOX4 Antibody, clone 10A8.1 is validated for use in Western Blotting, Immunohistochemistry for the detection of NOX4.

Quality

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NOX4 in 10 µg of human kidney tissue lysate.

Target description

~67 kDa observed. 66.93 kDa (isoform 1), 58.41 kDa (isoform 2; Nox4A), 64.10 kDa (isoform 8), 27.63 kDa (isoform 3; Nox4E), 31.81 kDa (isoform 4; Nox4D), 62.75 kDa (isoform 6; Nox4B) calculated.

Physical form

Mouse monoclonal IgG2bκ hybridoma culture supernatant without preservatives.

Unpurified.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.