ANTI-MERS-COV NUCLEOPROTEIN. CLONE 46 (C005B-450172)

General description

Platelet glycoprotein 4 (UniProt: Q08857; also known as Glycoprotein IIIb, GPIIIB, PAS IV, PAS-4, Platelet glycoprotein IV, GPIV, SR-B2 CD36) is encoded by the cd36 gene (Gene ID: 12491) in murine species. CD36 us a multi-pass membrane glycoprotein with two cytoplasmic domains (aa 1-7 and 462-472), two transmembrane domains (aa 8-29 and 440-461), and one extracellular domain (aa 30-439). It is highly expressed in intestine on the luminal surface of enterocytes. Its expression is also observed in the apical side of lingual taste bunds of circumvallate papillae. Its expression is also reported on monocyte-derived inflammatory macrophages that infiltrate into alveolar spaces, but on alveolar resident macrophages. It serves as a multifunctional protein and acts as a receptor for a broad range of ligands, such as thrombospondin, fibronectin, collagen, oxidized low-density lipoprotein, and bacterial diacylated lipopeptides. It is generally multivalent and can engage multiple receptors simultaneously, resulting in the formation of CD36 clusters that initiate signal transduction and internalization of receptor-ligand complexes. Cellular responses to its ligands are reported to be involved in angiogenesis, inflammatory response, fatty acid metabolism, and dietary fat processing in the intestine. CD36 is shown to bind long-chain fatty acids and facilitate their transport into cells, thereby promoting muscle lipid utilization, gut fat absorption, and adipose energy storage. It is also an important factor in both ventromedial hypothalamus neuronal sensing of long-chain fatty acid and the regulation of energy and glucose homeostasis. CD36 also reported to be a sensor for lipoteichoic acid, a Gram-positive cell wall component, and transmits inflammatory signals in conjunction with TLR2/TLR6 heterodimer and this cluster triggers signaling leading to NF-kB-dependent production of TNF-a via MYD88 signaling pathway. Clone ZND36-6 is an anti-SR-B2 (CD36) blocking antibody that has been used in pulmonary inflammation induced by exposure to silica. (Ref.: Tsugita, M., et al. (2017). Cell Rep. 18(5); 1298-1311; Stewart, CR., et al, (2010). Nat. Immunol. 11(2):155-161; Endemann, G., et al (1993). J. Biol. Chem. 268(16):11811-11816).

Specificity

Clone ZND36-6 is a rat monoclonal antibody that detects murine CD36 (SR-B2).

Immunogen

NIH3T3 cells expressing murine CD36 (SR-B2).

Application

Quality Control Testing

Evaluated by Immunocytochemistry in NIH 3T3 cells.

Immunocytochemistry Analysis: A 1:25 dilution of this antibody detected CD36 in NIH 3T3 cells.

Tested Applications

Flow Cytometry: A representative lot detected CD36 in Flow Cytometry applications (Tsugita, M., et al. (2017). Cell Rep. 18(5):1298-1311; Omori, S., et al. (2021). Cell Rep. 34(6):108734).

Inhibition Assay: A representative lot of this antibody partially inhibited IL-1b secretion by bone marrow derived macrophages. (Tsugita, M., et al. (2017). Cell Rep. 18(5):1298-1311).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-CD36, clone ZND36-6, Cat. No. MABF3056, is a rat monoclonal antibody that detects CD36 and is tested for use in Flow Cytometry, Immunocytochemistry, and Inhibition Assay.

Physical form

Purified rat monoclonal antibody IgG2a in PBS without azide.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.