ANTI-INTEGRIN ALPHAV ANTIBODY CLONE LM142 (C005B-452669)

General description

Integrin aV (UniProt: P06756; also known as Vitronectin receptor, Vitronectin receptor subunit alpha, CD51) is encoded by the ITGAV (also known as MSK8, VNRA, VNTR) gene (Gene ID: 3685) in human. Integrins are heterodimeric integral membrane proteins composed of an a subunit and a b subunit that function in cell surface adhesion and signaling. They contain a large extracellular domain responsible for ligand binding, a single transmembrane domain, and a cytoplasmic domain. The exact combination of various a- and b-subunits dictates the binding specificity of integrins to different ECM components. Although both subunits are required for adhesion, the binding specificity primarily depends on the extracellular portion of the a-subunit. The structural and functional diversity of the integrin family is based upon the pairing abilities of the individual a and b subunits. Integrins are not constitutively active and their activation from a low ligand-binding affinity state to high ligand-binding affinity state requires conformational change that can originate from either from their cytoplasmic or extracellular interactions. The recognition site for most integrins that bind the ECM consists of an RGD (arginine-glycine-aspartic acid) sequence. Integrin aV is a single-pass membrane glycoprotein that is synthesized with a signal peptide (aa 1-30), which is subsequently cleaved off to generate the mature form. The mature form contains an extracellular domain (aa 31-992), a transmembrane domain (aa 993-1016), and a cytoplasmic domain (aa 1017-1048). The a-subunit is composed of a heavy (aa 31-889) and a light (aa 891-1048) chain linked by a disulfide bond. Integrin aV can associate with b-1, b-3, b -5, b-6, or b-8 in a non-covalent manner. (Ref.: Cheresh, DA. (1987). Proc. Natl. Acad. Sci. USA. 84(18); 6471-6475).

Specificity

Clone LM142 is a mouse monoclonal antibody that detects human Integrin aV.

Immunogen

Purified aVb3 isolated from human placenta.

Application

Quality Control Testing

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: 1 μg of this antibody detected Integrin aV in one million HeLa cells.

Tested Applications

Surface plasmon resonance: A representative lot detected Integrin aV in Surface plasmon resonance applications (Chesnokova, L.S., et. al. (2011). J Virol. 85(24):13214-23).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin aV in Immunoprecipitation applications (Ueda, M., et. al. (2010). Biomaterials. 31(25):6394-9; Cheresh, D.A., et. al. (1987). Proc Natl Acad Sci USA. 84(18):6471-5).

Immunocytochemistry Analysis: A 1:25 dilution from a representative lot detected Integrin aV in A431 cells.

Immunofluorescence Analysis: A representative lot detected Integrin aV in Immunofluorescence applications (Straub, S., et. al. (2013). PLoS One. 8(1):e53309).

Affinity Binding Assay: A representative lot detected Integrin aV in Affinity Binding Assay applications (Kapp, T.G., et. al. (2017). Sci Rep. 7:39805).

Flow Cytometry Analysis: A representative lot detected Integrin aV in Flow Cytometry applications (Borbely, A., et. al. (2019). Pharmaceutics. 11(4):151).

Immunocytochemistry Analysis: A representative lot detected Integrin aV in Immunocytochemistry applications (Cheresh, D.A., et. al. (1987). Proc Natl Acad Sci USA. 84(18):6471-5).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Integrin aV, clone LM142, Cat. No. MAB1978-I, is a mouse monoclonal antibody that detects Integrin aV and is used in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Surface plasmon resonance.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.