ANTI-CYCLIN E ANTIBODY CLONE HE12 (C005B-243935)

General description

Cyclin E is a regulatory subunit of Cdk2 and controls G1 / S transition during the mammalian cell cycle. Multiple isoforms of Cyclin E are only expressed in tumors but not in normal tissue, suggesting a post transcriptional regulation of Cyclin E. In vitro analyses indicated that these truncated variant isoforms of Cyclin E are able to phosphorylate histone H1. Alterations in the Cyclin E protein have been implicated as indicators of worse prognosis in various cancers.

Specificity

human p52cyclin E

Immunogen

Recombinant human cyclin E

Application

Anti-Cyclin E Antibody, clone HE12 is a high quality Mouse Monoclonal Antibody for the detection of Cyclin E & has been validated in IP, WB, ICC.

Quality

routinely evaluated in immunoblot on human A431 lysate

Target description

52 kDa

Linkage

Replaces: 04-223

Physical form

Format: Purified

Storage and Stability

2 years at -20°C or 6 months at 4°C

Analysis Note

Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.

Legal Information

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