AMIDO BLACK STAINING SOLUTION 2X ELECTROPHORESIS REAGENT

General description

Amido black (AB) is an acidic dye used for staining proteins. It can detect proteins levels of 50ng in both polyvinylidene difluoride (PVDF) and nitrocellulose membrane. AB aids protein visualization even at low concentrations. It has applications in forensics due to its ability to stain blood proteins in fingerprints.

Application

Amido Black Staining Solution 2X has been used in the staining:

• serum proteins present in the culture supernatant
• cysteine sulfinic acid decarboxylase protein in mouse liver homogenate
• histones from chromatin samples

Reconstitution

After dilution with deionized water to a final volume of 500 ml, solution will contain 0.1% (w/v) amido black, 25% (v/v) isopropanol and 10% (v/v) acetic acid.

Molecular Weight: 616.49. Empirical Formula: C₂₂H₁₄N₆Na₂O₉S₂. Quality Level: 200. form: liquid. SMILES string: [Na+].[Na+].Nc1c(\N: N/c2ccc(cc2)[N+]([O-]): O)c(cc3cc(c(\C: C/c4ccccc4)c(O)c13)S([O-])(: O): O)S([O-])(: O): O. InChI: 1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31. . /h1-11,29H,23H2,(H,32,33,34)(H,35,36,37). . /q. 2*+1/p-2/b26-25+,27-24+. . . InChI key: AOMZHDJXSYHPKS-DROYEMJCSA-L. Pictograms: GHS02,GHS07. Signal Word: Danger. Hazard Statements: H225 - H315 - H319 - H336. Precautionary Statements: P210 - P233 - P240 - P241 - P303 + P361 + P353 - P305 + P351 + P338. Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 3. Target Organs: Central nervous system. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 53.6 °F. Flash Point(C): 12 °C.