A-CHYMOTRYPSIN TYPE II FROM BOVINE PANCREAS (C005B-002505)

Application

The enzyme from Sigma has been used to study the structure-function relationship in glycosylated α-chymotrypsin using immobilized metal-ion affinity chromatography (IMAC) and immobilized metal-ion affinity capillary electrophoresis (IMACE).

Biochem/physiol Actions

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. The pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, and α2-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

Unit Definition

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

Preparation Note

Produced from 3× crystallized chymotrypsinogen

Analysis Note

Protein determined by E1%/280

Other Notes

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

type: Type II. Quality Level: 300. form: lyophilized powder. specific activity: ≥. 40 . units/mg protein. mol wt: 25 . kDa. composition: protein, ≥. 85% . color: white to off-white. UniProt accession no.: P00767. application(s): diagnostic assay manufacturing. storage temp.: −. 20°C. Gene Information: cow ... CTRB1(618826). Pictograms: GHS07,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H302 - H315 - H319 - H334 - H335 - H400. Precautionary Statements: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.