A-AMYLASE TYPE II-A FROM BACILLUS SPECIES (C005B-087420)

General description

α-Amylase, an extracellular enzyme, is present in many animals and plants, and also in microorganisms, such as different Bacillus species.

Application

α-Amylase from Bacillus sp. has been used:

• as a component of salivary fluid to perform artificial mastication; in luminal gastrointestinal digestion experiment
• as a standard in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to determine the concentration of α-Amylase
• to de-starch the alcohol insoluble residue (AIR) for non-cellulosic neutral monosaccharide analysis

Biochem/physiol Actions

α-Amylase degrades starch into oligosaccharides such as maltose, and glucose and alpha limit dextrin. It hydrolyzes the α-(1→4) glucosidic linkages in polysaccharides of three or more α-(1→4) linked D-glucose units, without hydrolyzing the α-(1→6) bond. It participates in glucose production and is essential for energy acquisition. α-Amylases are widely known industrial enzymes used in the food, detergent, textile, fermentation, and pharmaceutical industries.

Features and Benefits

Maintains >98% of activity after 60 minutes at pH 6.2 at 85 °C.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 Â°C.

Preparation Note

4× crystallized

Dissolves in water to form a clear, colorless solution at 0.1 mg/mL concentration.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

type: Type II-A. Quality Level: 200. form: lyophilized powder. specific activity: ≥. 1,500 . units/mg protein (biuret). mol wt: 50-55 . kDa by SDS-PAGE. solubility: H₂O: soluble 0.1 . mg/mL, clear, colorless. storage temp.: −. 20°C. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H334. Precautionary Statements: P261 - P284 - P501. Hazard Classifications: Resp. Sens. 1. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Faceshields, Gloves.