AGAROSE LE 500G (C005B-225755)

General description

Agarose is obtained from seaweed and contains repeated agarobiose units. During agarose gel formation, the polymers aggregate to form a network with varying pore sizes. This property is used in agarose gel electrophoresis to separate DNA.

Application

Agarose LE has been used for:

• analyzing fragments between 0.2 and 15 kb
• analysis of PCR products
• examination of restriction endonucleases
• digests of plasmid, cosmid, and λ phage DNA
• electrophoresis of RNA in, e.g., denaturing gels containing formaldehyde
• in combination with ethidium bromide ​in DNA electrophoresis to facilitate the separation and visualization of DNA fragments.

Nucleic acid fragments separated with Agarose LE can be blotted to nylon or nitro-cellulose membranes by all standard blotting techniques.
Important Note: Detection with nonradioactive probes e.g., digoxigenin-labeled nucleic acids, does not interfere with the use of Agarose LE.

Quality

Absence of DNase: none detected according to the current Quality Control procedures.
Absence of RNase: none detected according to the current Quality Control procedures.

Other Notes

For life science research only. Not for use in diagnostic procedures.

biological source: algae (seaweed). form: powder. packaging: pkg of 100 . g (11685660001), pkg of 500 . g (11685678001). manufacturer/tradename: Roche. EEO: 0.05 -0.13. transition temp: gel point 36 . °C ±1.5. gel strength: >: 2,500 . g/cm². foreign activity: Dnase, none detected, Rnase, none detected. shipped in: ambient. storage temp.: 20-25°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): does not flash. Flash Point(C): does not flash.