AGAR WASHED--PLANT CELL CULTURE TESTED (C005B-087798)

General description

Agar or Agar-agar is one of the major components found in the cell wall of red algae. It is a polysaccharide complex, comprising about 70% agarose and 30% agaropectin.

Application

Washed agar has been used:

• as a supplement (0.9% w/v) to Murashige and Skoog basal medium for plant growth
• in the preparation of cocultivation medium for Agrobacterium tumefaciens mediated transformation in maize (Zea mays) hybrid Hi-II
• as a component in the complete medium to regenerate protoplasts of Piriformospora indica
• as a clean agar medium to culture Synechococcus WH7803 strain

Biochem/physiol Actions

Agar is an ester of sulfuric acid in a linear galactan form, that makes it soluble in hot water and insoluble in cold. Washed agar contain less impurities and low level of nutrients.

Preparation Note

Washed

linearFormula: (C₁₂H₁₈O₉)_n. biological source: algae (Rhodophyceae). Quality Level: 200. form: powder. technique(s): cell culture | plant: suitable. mp: 80-90 . °C (1.5% in water). transition temp: transition temp 27-37 . °C (1.5%). application(s): agriculture. storage temp.: room temp. InChI: 1S/C14H24O9/c1-5-8(16)13-11(7(21-5)4-20-13)23-14-10(18)12(19-2)9(17)6(3-15)22-14/h5-18H,3-4H2,1-2H3/t5?,6-,7?,8-,9+,10-,11?,12+,13+,14?/m1/s1. InChI key: GYYDPBCUIJTIBM-DYOGSRDZSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).