AF CHOLESTEROL ASSAY KIT

General description

Cholesterol is a sterol and lipid present in the cell membranes, and is transported in the bloodstream of all animals. It is used to form cell membranes and hormones, and plays important roles in cell signaling processes. Elevated levels (hypercholesterolemia) have been associated with cardiovascular diseases such as atherosclerosis; whereas, low levels (hypocholesterolemia) may be linked to depression, cancer and cerebral hemorrhage.

Features and Benefits

• Linear detection range in 96-well plate: 0.1 to 10 mg/dL cholesterol for colorimetric assay and 0.02 to 2 mg/dL for fluorimetric assay.
• Room temperature assay.
• Compatible with high-throughput handling systems.

Suitability

The kit is suitable for the quantitative determination of cholesterol and evaluation of drug effects on cholesterol metabolism in serum, plasma etc.

Principle

Simple, direct and automation-ready procedures for measuring cholesterol are very desirable. The AF Cholesterol Assay Kit uses a single working reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at A₅₇₀ nm or fluorescence intensity at λ_Ex = 530 nm/λ_Em = 585 nm is directly proportional to the total cholesterol concentration in the sample.