ACRYLAMIDE ELECTROPHORESIS REAGENT (C005B-080860)

General description

Acrylamide functions as a quencher of tryptophan fluorescence. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules. Bis-acrylamide is essential for crosslinking and acrylamide is required for the polymerization of monomers. Acrylamide alkylates SH groups of cysteine and ε-NH₂ lysine residues of proteins during electrophoresis.

Application

Acrylamide has been used in the preparation of polyacrylamide gels.

Compatibility

This substance has been tested against several types of hand protection for CE compliance. See Related Products to find the recommended gloves for handling this product.

Molecular Weight: 71.08. Empirical Formula: C₃H₅NO. linearFormula: CH₂: CHCONH₂. vapor density: 2.45 (vs air). Quality Level: 200. vapor pressure: 0.03 mmHg ( 40 °C). Assay: ≥. 99% (HPLC). form: powder. technique(s): electrophoresis: suitable. impurities: ≤. 0.005% Acrylic acid, ≤. 0.005% Insoluble in water. conductivity: ≤. 10 . μ. mho (35% solution). bp: 125 . °C/25 . mmHg (lit.). mp: 82-86 . °C (lit.). solubility: water: soluble 2.5 . g/10 mL, clear, colorless. cation traces: Fe: ≤. 1 . ppm, Mg: ≤. 3 . ppm, heavy metals (as Pb): ≤. 5 . ppm. SMILES string: NC(: O)C: C. InChI: 1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5). InChI key: HRPVXLWXLXDGHG-UHFFFAOYSA-N. Pictograms: GHS06,GHS08. Signal Word: Danger. Hazard Statements: H301 - H312 + H332 - H315 - H317 - H319 - H340 - H350 - H361f - H372. Precautionary Statements: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral. Target Organs: Peripheral nervous system. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): 280.4 °F - closed cup. Flash Point(C): 138 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges.