ACETYL-COA 200MG

General description

Acetyl-Coenzyme A, trilithium salt

Application

Acetyl-Coenzyme A is used to assay CAT enzyme activity in cell extracts using radioisotopes. CAT enzyme activity in cell extracts catalyzes the transfer of acetyl groups from acetylcoenzyme A to chloramphenicol. A number of assays have been developed to measure CAT activity in cell extracts. Acetyl-Coenzyme A has also been used to determine citrate synthase activity.

Biochem/physiol Actions

Acetyl-Coenzyme A (Ac-CoA) is the end product of glycolysis and takes part in the Ac-CoA pathway, which is a metabolic pathway for carbon compounds. Ac-CoA is important in cholesterol synthesis. It also takes part in fatty acid biosynthesis and catabolism of polyamines like spermine and spermidine. A low level of Ac-CoA leads to a loss in glial cell and neuronal function. Ketone bodies and triglycerides give rise to Ac-CoA on hydrolysis and this indirectly leads to increased histone acetylation.

Preparation Note

Working solution: Optimal solvent: water or aqueous solution with a weak acidic pH (4 to 5).
Storage conditions (working solution): -15 to -25 °C
50 mg/ml in phosphate buffer, pH 7, is stable for 3 weeks at -15 to -25 °C. Unfrozen solutions should be immediately used.

Other Notes

For life science research only. Not for use in diagnostic procedures.

description: Chemical Formula: C₂₃H₃₅N₇O₁₇P₃SLi₃. Quality Level: 100. Assay: 85% (Enzymatic and Absorbance). form: solid. mol wt: M_r 809.6 (acetyl-CoA), M_r 827.4 (acetyl-CoA-Li₃). packaging: pkg of 010 . mg (10101893001), pkg of 200 . mg (11585371001), pkg of 050 . mg (10101907001). manufacturer/tradename: Roche. concentration: 2% (lithium). storage temp.: 2-8°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.