ACES >= 99.0% (TITRATION) (C005B-059379)

General description

N-(2-Acetamido)-2-aminoethanesulfonic acid (ACES) is a zwitterionic buffer used in biochemistry and molecular biology research. It is one of the Good′s buffers with a pH range of 6.15 - 8.35. The preferred range of ACES buffer in an aqueous solution is 6.1 - 7.5.

Application

ACES has been used:

• as a component of buffered charcoal yeast extract (BYCE) agar for plating bacteria culture and Buffered Yeast Extract (BYE) liquid medium to culture bacteria
• to study the impact of the pH shift on the thermal inactivation of bacterial (Bacillus subtills) spores
• as a buffer system to study the depolymerization of starch by α-amylase during exposure to hydrostatic pressure.

It is also suitable for use: as buffers for both agarose and polyacrylamide gel electrophoresis; in the isoelectric focusing of proteins; in the analysis of bacterial autolysins in a discontinuous sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) system.

Other Notes

The product is intended for laboratory use only, and it is not suitable for drug, household, or other purposes.

Molecular Weight: 182.20. Empirical Formula: C₄H₁₀N₂O₄S. linearFormula: H₂NCOCH₂NHCH₂CH₂SO₃H. Quality Level: 200. Assay: ≥. 99.0% (titration). form: crystalline powder. pH: 3.0-4.5. useful pH range: 6.1-7.5. pKa (25 °C): 6.8. mp: >. 220 . °C (dec.) (lit.). solubility: water: 0.523 . g/mL, clear, colorless. application(s): diagnostic assay manufacturing. storage temp.: room temp. SMILES string: NC(: O)CNCCS(O)(: O): O. InChI: 1S/C4H10N2O4S/c5-4(7)3-6-1-2-11(8,9)10/h6H,1-3H2,(H2,5,7)(H,8,9,10). InChI key: DBXNUXBLKRLWFA-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, type N95 (US).