7-AMINOACTINOMYCIN D (C005B-058903)

General description

7-Aminoactinomycin D (7-ADD), an analog of actinomycin D (AD) is a membrane-impermeable red fluorescent DNA dye. It can selectively bind GC regions of DNA. 7-ADD is used in flow cytometry to differentiate between viable, apoptotic, and late apoptotic/dead cells. This DNA stain finds its application in chromosome analysis, cell cycle studies, and quantification of apoptosis.

Chemical structure: peptide

Application

7-Aminoactinomycin D has been used:

• for nuclei visualization in mouse tail skin
• as a nuclear stain in imaging flow cytometry for nucleus-protein colocalization
• in flow cytometry to determine the level of CD16 (Fc gamma III receptor) and CD32 (Fc gamma II receptor) in epidermal cells

Features and Benefits

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Molecular Weight: 1270.43. Empirical Formula: C₆₂H₈₇N₁₃O₁₆. Assay: ~97% (HPLC). Quality Level: 300. form: powder. color: red to very dark purple. antibiotic activity spectrum: neoplastics. Mode of action: DNA synthesis | interferes. storage temp.: 2-8°C. SMILES string: CC(C)[C@H]1NC(: O)[C@@H](NC(: O)c2cc(N)c(C)c3OC4: C(C)C(: O)C(N): C(C(: O)N[C@H]5[C@@H](C)OC(: O)[C@@H](C(C)C)N(C)C(: O)CN(C)C(: O)[C@@H]6CCCN6C(: O)[C@H](NC5: O)C(C)C)C4: Nc23)[C@@H](C)OC(: O)[C@H](C(C)C)N(C)C(: O)CN(C)C(: O)[C@@H]7CCCN7C1: O. InChI: 1S/C62H87N13O16/c1-26(2)42-59(85)74-21-17-19-36(74)57(83)70(13)24-38(76)72(15)48(28(5)6)61(87)89-32(11)44(55(81)66-42)68-53(79)34-23-35(63)30(9)51-46(34)65-47-40(41(64)50(78)31(10)52(47)91-51)54(80)69-45-33(12)90-62(88)49(29(7)8)73(16)39(77)25-71(14)58(84)37-20-18-22-75(37)60(86)43(27(3)4)67-56(45)82/h23,26-29,32-33,36-37,42-45,48-49H,17-22,24-25,63-64H2,1-16H3,(H,66,81)(H,67,82)(H,68,79)(H,69,80)/t32-,33-,36+,37+,42-,43-,44+,45+,48+,49?/m1/s1. InChI key: YXHLJMWYDTXDHS-SGILFZQNSA-N. Pictograms: GHS06. Signal Word: Danger. Hazard Statements: H300. Precautionary Statements: P301 + P330 + P331 + P310. Hazard Classifications: Acute Tox. 2 Oral. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges.