6-DIAZO-5-OXO-L-NORLEUCINE (C005B-020292)

General description

Chemical structure: amino acid derivatives

Application

6-Diazo-5-oxo-L-norleucine (DON) has been used as an inhibitor of glutamine synthetase–glutamine(amide)-2-oxoglutarate aminotransferase.

Biochem/physiol Actions

6-Diazo-5-oxo-L-norleucine (DON), a glutamine analogue, is used as an inhibitor to study the function, specificity and characteristics of cytidine triphosphate synthase 1(s) (CTPS1) and various glutaminase(s). DON is used to inhibit glutamine (GLN) flux between cells.

DON is associated with a number of reactions involving L-glutamine as a nitrogen source, such as nucleic acid and protein synthesis. It is known to possess anticancer activity against choriocarcinoma.

DON is used to study mechanisms of glutamine utilizing enzymes such as carbamoyl phosphate synthase and cytidine triphosphate synthase.

Molecular Weight: 171.15. Empirical Formula: C₆H₉N₃O₃. Assay: >. 95% (TLC). Quality Level: 200. form: crystalline. technique(s): ligand binding assay: suitable. color: light yellow. mp: 145 . °C. antibiotic activity spectrum: neoplastics. Mode of action: enzyme | inhibits. storage temp.: −. 20°C. SMILES string: N[C@@H](CCC(: O)C: [N+]: [N-])C(O): O. InChI: 1S/C6H9N3O3/c7-5(6(11)12)2-1-4(10)3-9-8/h3,5H,1-2,7H2,(H,11,12)/t5-/m0/s1. InChI key: YCWQAMGASJSUIP-YFKPBYRVSA-N. Pictograms: GHS06. Signal Word: Danger. Hazard Statements: H301 + H311 + H331. Precautionary Statements: P261 - P264 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P311. Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral. Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges.