General description

This Certified Spiking Solution^® is suitable for GC/MS and LC/MS applications from clinical and diagnostic testing and endocrinology to sports testing.

5α-Dihydrotestosterone (DHT) is a steroid hormone and metabolite of the natural androgen, testosterone, formed by the action of NADPH-dependent enzyme 5α-reductase. It has been implicated in a broad range of diseases from male pattern baldness to benign prostatic hyperplasia and prostate cancer. As an anabolic steroid, DHT has also been used as a performance-enhancing drug to promote muscle growth.

Application

The Certified Reference Material (CRM) in solution can also be used as follows:

Simultaneous analysis of testosterone and 5α-dihydrotestosterone using a benchtop gas chromatograph-mass spectrophotometer (GC-MS) in human serum samples
Liquid chromatography-electrospray ionization-tandem mass spectrometric (LC/ESI-MS/MS) determination of testosterone and 5α-dihydrotestosterone following their proton affinitive derivatization using picolinic acid and its analogs
Determination of testosterone and 5α-dihydrotestosterone in plasma and testis samples of fathead minnow by a gas chromatography-tandem mass spectrometric (GC-MS/MS) method
Multi-residue screening of epitestosterone, testosterone, 5α-dihydrotestosterone, dehydroepiandrosterone, androsterone, and etiocholanolone in urine samples by high-resolution full scan-electrospray ionization-liquid chromatography (HR-FS-ESI-LC) combined with quadrupole orbitrap mass spectrometer (Q/Orbitrap MS) following requirements of World Antidoping Agency (WADA)
Quantitative analysis of nine hydroxy-androgens and their conjugates by a stable isotope dilution liquid chromatography-electrospray ionization-tandem mass spectrometry (SID-LC-ESI-MS/MS) based method following their derivatization using picolinic acid

Features and Benefits

Fully characterized under ISO/IEC 17025 and ISO 17034 accreditation
Accompanied with a comprehensive Certificate of Analysis (CoA) with data on stability, homogeneity, accuracy of concentration, uncertainty, and traceability
Rigorously tested through real-time stability studies to ensure accuracy and shelf life
Gravimetrically prepared using qualified precision balances to ensure minimal uncertainty
Flame sealed under argon into ampoules for long-term shelf life
Offered in a convenient, DEA-exempt format to improve laboratory efficiency

Legal Information

CERILLIANT is a registered trademark of Cerilliant Corporation

CERTIFIED SPIKING SOLUTION is a registered trademark of Cerilliant Corporation

Snap-N-Shoot is a registered trademark of Cerilliant Corporation

Snap-N-Spike is a registered trademark of Cerilliant Corporation

Molecular Weight: 290.44. Empirical Formula: C₁₉H₃₀O₂. grade: certified reference material. Quality Level: 300. form: liquid. feature: Snap-N-Spike^®./Snap-N-Shoot^®.. packaging: ampule of 1 . mL. manufacturer/tradename: Cerilliant^®.. concentration: 1.0 . mg/mL in methanol. technique(s): gas chromatography (GC): suitable, liquid chromatography (LC): suitable. application(s): clinical testing
clinical testing. format: single component solution. storage temp.: −. 70Â°C. SMILES string: C[C@]12CC[C@H]3[C@@H](CC[C@H]4CC(: O)CC[C@]34C)[C@@H]1CC[C@@H]2O. InChI: 1S/C19H30O2/c1-18-9-7-13(20)11-12(18)3-4-14-15-5-6-17(21)19(15,2)10-8-16(14)18/h12,14-17,21H,3-11H2,1-2H3/t12-,14-,15-,16-,17-,18-,19-/m0/s1. InChI key: NVKAWKQGWWIWPM-ABEVXSGRSA-N. Pictograms: GHS02,GHS06,GHS08. Signal Word: Danger. Hazard Statements: H225 - H301 + H311 + H331 - H370. Precautionary Statements: P210 - P233 - P280 - P301 + P310 - P303 + P361 + P353 - P304 + P340 + P311. Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Flam. Liq. 2 - STOT SE 1. Storage Class Code: 3 - Flammable liquids. WGK: WGK 2. Flash Point(F): 49.5 Â°F - closed cup. Flash Point(C): 9.7 Â°C - closed cup.