Application
Isoamyl alcohol (3-methylbutanol) may be used as a protein denaturation component of various RNA and DNA extraction and purification protocols. Isoamyl alcohol is frequently used together with phenol and or chloroform in nucleic acid preparation protocols.
Other Notes
Reduces interface between 2 phases
Molecular Weight: 88.15. Empirical Formula: C
5H
12O. linearFormula: (CH
3)
2CHCH
2CH
2OH. grade: for molecular biology. Quality Level: 100. product line: BioUltra. Assay: ≥. 99.0% (GC). form: liquid. technique(s): DNA extraction: suitable, RNA extraction: suitable. impurities: DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤. 0.01% free acid (as CH
3COOH). evapn. residue: ≤. 0.003%. refractive index:
n20/D 1.407. pH: 5.6 (20 . °C, 25 . g/L). density: 0.809 . g/mL . at 20 . °C (lit.). cation traces: Al: ≤. 0.5 . mg/kg, Ba: ≤. 0.1 . mg/kg, Bi: ≤. 0.1 . mg/kg, Ca: ≤. 0.5 . mg/kg, Cd: ≤. 0.05 . mg/kg, Co: ≤. 0.02 . mg/kg, Cr: ≤. 0.02 . mg/kg, Cu: ≤. 0.02 . mg/kg, Fe: ≤. 0.1 . mg/kg, K: ≤. 0.5 . mg/kg, Li: ≤. 0.1 . mg/kg, Mg: ≤. 0.1 . mg/kg, Mn: ≤. 0.02 . mg/kg, Mo: ≤. 0.1 . mg/kg, Na: ≤. 0.5 . mg/kg, Ni: ≤. 0.02 . mg/kg, Pb: ≤. 0.1 . mg/kg, Sr: ≤. 0.1 . mg/kg, Zn: ≤. 0.1 . mg/kg. λ: neat. UV absorption: λ. : 260 . nm A
max: 0.06, λ. : 280 . nm A
max: 0.06. SMILES string: CC(C)CCO. InChI: 1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3. InChI key: PHTQWCKDNZKARW-UHFFFAOYSA-N. Pictograms: GHS02,GHS05,GHS07. Signal Word: Danger. Hazard Statements: H226 - H315 - H318 - H332 - H335. Precautionary Statements: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P312 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3. Target Organs: Respiratory system. Storage Class Code: 3 - Flammable liquids. WGK: WGK 1. Flash Point(F): 110.3 °F - closed cup. Flash Point(C): 43.5 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter.