3,3′,5,5′-Tetramethylbenzidine dihydrochloride (C007B-566526)

3,3′,5,5′-Tetramethylbenzidine dihydrochloride has been used as a substrate for theanti-mouse IgG (? specific) horseradish peroxidase-conjugated antibody in the ELISA of mice seraanti-mouse peroxidase antibody in the protein tyrosine phosphatase assay of naive and effector T cell lysatein enzymatic hydroperoxide (EH) assay and in prooxidants–antioxidants balance (PAB) assay of serum samples from diabetic patients3,3′,5,5′-Tetramethylbenzidine (TMB) is a peroxidase substrate suitable for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm.

Specification: tablet, 1 mg substrate per tablet

MDL Number: MFCD00012961

Molecular Formula: C16H20N2·2HCl

Molecular Weight: 313.27

EC Number: 264-769-6

PubChem CID: 174033

Isomeric SMILES: CC1=CC(=CC(=C1N)C)C2=CC(=C(C(=C2)C)N)C.Cl.Cl