Name: 2' 7'-DICHLOROFLUORESCEIN SUITABLE FOR FLUORESCENCE BIOREAGENT >= 90% (T) (C005B-078279)
SKU: C005B-078279
Availability: OutOfStock

2' 7'-DICHLOROFLUORESCEIN SUITABLE FOR FLUORESCENCE BIOREAGENT >= 90% (T) (C005B-078279)

Catalog No.

C005B-078279

Manufacturer No.

35848-5G

Manufacturer Name

Sigma-Aldrich

Quantity

Unit of Measure

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm.

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Description
Specifications

General description

2′,7′-Dichlorofluorescein, also known as DCF is a fluorescent dye. The DCF fluorescence excitation is at 498nm with emission at 522nm. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.

Application

2′,7′-Dichlorofluorescein is used as fluorescent probe to measure ROS (Reactive Oxygen Species) for biological studies. It may also be used as a dip reagent for the fluorometric determination of vicinal diols, glycosides, and phenols.

Molecular Weight: 401.20. Empirical Formula: C₂₀H₁₀Cl₂O₅. product line: BioReagent. Quality Level: 100. Assay: ≥. 90% (T). form: solid. mp: 280 . Â°C (dec.) (lit.). fluorescence: λ. _ex . 504 . nm. λ. _em . 529 . nm in 0.1 M Tris pH 8.0. suitability: suitable for fluorescence. SMILES string: Oc1cc2Oc3cc(O)c(Cl)cc3C4(OC(: O)c5ccccc45)c2cc1Cl. InChI: 1S/C20H10Cl2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H. InChI key: VFNKZQNIXUFLBC-UHFFFAOYSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves.