100 MM IPTG SOLUTION 15 ML

Application

100 mM IPTG Solution - Novagen has been used to:

• induce expression of an anti-CD11b-nanobody with a sortase recognition tag in Chalmers (WK6) cells
• induce expression in calcium-independent sortase from BL21(DE3) cells
• turn on bacterial protein production of the protein A/G-MNase

Biochem/physiol Actions

Isopropyl-β-D-1-thiogalactopyranoside (IPTG) is an inducer of the lacoperon. It is highly recommended for blue/white screening using lacZα-complementation in appropriate vectors and host strains, for example, pSTBlue-1/NovaBlue. In addition, it is also used for inducing protein expression with the pET system and various lac promoter-derived expression systems. IPTG is supplied as a sterile aqueous solution and is free of dioxane. It comes in convenient volumes of 10 × 1.5 ml. IPTG is used in bacterial cultures to induce the expression of plasmid-based genes to produce recombinant proteins under the control of the lac promoter.

Warning

Toxicity: Carcinogenic / Teratogenic (D)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Quality Level: 100. form: solution. mol wt: 238.30  . g/mol. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. shipped in: wet ice. storage temp.: −. 20°C. InChI: 1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1. InChI key: BPHPUYQFMNQIOC-NXRLNHOXSA-N. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.