1KB DNA LADDER FOR DNA ELECTROPHORESIS

General description

Sigma′s 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb, 1 kb repeats from 3 to 6 kb, and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis, five μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide, SYBR^® Green, and Nancy-520.

Application

1 kb DNA Ladder has been used as a molecular marker to determine the molecular weight and size of double stranded DNA during gel electrophoresis.

Components

Sigma′s 1kb Ladder is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Other Notes

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Legal Information

SYBR is a registered trademark of Life Technologies

Quality Level: 100, 200. form: liquid. suitability: suitable for electrophoresis (DNA). web metadata keyword.default: Ladder, Marker, NA electrophoresis. storage temp.: −. 20°C. Storage Class Code: 10 - Combustible liquids. Flash Point(F): Not applicable. Flash Point(C): Not applicable. Personal Protective Equipment: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US).