1-CHLORO-2 4-DINITROBENZENE 97% (C005B-091929)

Application

1-Chloro-2,4-dinitrobenzene was used in determination of relative amounts of individual glutathione S-transferases subunits in human tissues by reverse-phase HPLC. It was used as contact sensitizer to study the antibacterial peptides hBD-2, -3 and -4 and LL-37 induced IL-18 protein release in human keratinocytes.

Biochem/physiol Actions

2,4-dinitrochlorobenzene induces atopic dermatitis in BALB/c mice model.

Preparation Note

The product is dissolved in ethanol when used as glutathione S-transferase substrate and then added to a buffer to form a final concentration of 1mM in phosphate buffer (8 mL of 0.2 M KH₂PO₄ and 28 mL of 0.1 M Na₂HPO₄ per 100 mL of solution, adjusted to pH 6.5) and 4% (v/v) ethanol.

Molecular Weight: 202.55. Empirical Formula: C₆H₃ClN₂O₄. linearFormula: ClC₆H₃(NO₂)₂. Quality Level: 100. Assay: 97%. form: solid. expl. lim.: 22 . %. bp: 315 . °C (lit.). mp: 48-50 . °C (lit.). solubility: alcohol: soluble (hot), alcohol: very slightly soluble (cold), benzene: soluble, carbon disulfide: soluble, diethyl ether: soluble, water: insoluble. SMILES string: [O-][N+](: O)c1ccc(Cl)c(c1)[N+]([O-]): O. InChI: 1S/C6H3ClN2O4/c7-5-2-1-4(8(10)11)3-6(5)9(12)13/h1-3H. InChI key: VYZAHLCBVHPDDF-UHFFFAOYSA-N. Pictograms: GHS05,GHS06,GHS08,GHS09. Signal Word: Danger. Hazard Statements: H301 + H331 - H310 - H315 - H317 - H318 - H373 - H410. Precautionary Statements: P262 - P273 - P280 - P301 + P310 - P302 + P352 + P310 - P305 + P351 + P338. Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 2. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 2. Flash Point(F): 381.2 °F - closed cup. Flash Point(C): 194 °C - closed cup. Personal Protective Equipment: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges.