Singlet Oxygen Sensor Green Reagent

The SOSG (Singlet Oxygen Sensor Green Fluorescent Probe) produced by Aladdin, also known as Singlet Oxygen Sensor Green, Singlet Oxygen Sensor Green Reagent, or Singlet Oxygen Sensing Green Fluorescent Probe, is a highly selective green fluorescent probe for singlet oxygen (1O2) but lacks cell membrane permeability. It is commonly used for detecting singlet oxygen in solution systems and evaluating the efficacy of free radical scavengers. SOSG singlet oxygen fluorescent probe has high selectivity for 1O2; Unlike other fluorescent or chemiluminescence singlet oxygen detection reagents, it has no significant response to hydroxyl radicals (• OH) and superoxide ion radicals (• O2-). This new singlet oxygen indicator has weak blue fluorescence, with excitation peaks at 372 and 393nm and emission peaks at 395 and 416nm. When singlet oxygen is present, the probe can emit green fluorescence similar to fluorescein (Ex/Im: 504/525nm). The SOSG singlet oxygen fluorescent probe reagent provides a non cell permeable derivative.The SOSG singlet oxygen fluorescence probe can be used for the detection of 1O2 in aqueous solutions and can also be used to evaluate the efficacy of free radical scavengers, which are commonly used to improve the flavor and nutritional quality of food. It should be noted that the SOSG singlet oxygen fluorescent probe will undergo activation under alkaline pH conditions or in certain solvents, including but not limited to acetonitrile, DMSO, DMF, acetone, etc. Over time, its fluorescent products may also degrade in certain solutions, but if the conditions are properly controlled, the intensity of the green fluorescence signal can be correlated with the concentration of 1O2 without interference from other reactive oxygen species.Precautions：1.Fluorescent dyes are prone to quenching, so please avoid exposure to light to minimize fluorescence quenching.We recommend using this product in a sterile environment to ensure stable and consistent assay results.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.2.After receiving the product, immediately dry it and store it in a refrigerator at ≤ -20°C away from light; If long-term storage is required, it is recommended to store in a -80℃ refrigerator. Remove it before use.Instructions for Use：1.Preparation of SOSG Stock Solution in methanol: Dissolve 100µg of this product in 33µl of methanol to make a stock solution of approximately 5mM . Note: Aliquot and store the SOSG Stock Solution at -80ºC, and use within one month.2.Preparation of SOSG Working Solution: The SOSG Working Solution should be prepared immediately before use in aqueous solution, such as ultrapure water or 100mM Tris (pH 7.5). The optimal working concentrations vary depending on experiments, with a suggested starting concentration range of 1-10µM.3.Any excess SOSG working solution should be discarded, and can not be kept for later use.

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