ISOLATED MITOCHONDRIA STAINING KIT FOR DETECTION OF MITOCHONDRIAL POTENTIAL CHANGES

Application

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.

Features and Benefits

• Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
• Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
• Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

Principle

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

Analysis Note

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

Quality Level: 200. usage:  . kit sufficient for 1000 . reactions (using 96 multiwell plates),  . kit sufficient for 50 . reactions (in a 2 mL cuvette). packaging: pkg of 1 . kit. storage condition: dry at room temperature. technique(s): flow cytometry: suitable, protein staining: suitable. fluorescence: λ. _ex . 490 . nm. λ. _em . 590 . nm (JC-1 dye). application(s): cell analysis
detection. detection method: fluorometric. shipped in: dry ice. storage temp.: −. 20°C. Pictograms: GHS06. Signal Word: Danger. Hazard Statements: H300 + H310. Precautionary Statements: P262 - P264 - P280 - P301 + P310 - P302 + P352 + P310 - P361 + P364. Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Oral. Storage Class Code: 6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.