ANTI-ING3 CLONE 2A2

General description

Inhibitor of growth protein 3 (UniProt: Q9NXR8; also known as ING3, p47ING3) is encoded by the ING3 (also known as HSPC301) gene (Gene ID 54556) in human. ING3 is a ~50 kDa protein that belongs to a family of proteins that contain PHD-finger domain and act as readers of the epigenetic code through specific recognition of trimethylated lysine 4 of histone H3. ING3 is a component of the NuA4 histone acetyltransferase (HAT) complex, which is involved in transcriptional activation of select genes, principally by acetylation of nucleosomal histones H4 and H2A. This complex is also shown to activate p53 trans-activated promoters, including promoters of p21/waf1 and bax. ING3 is highly expressed in small intestine, bone marrow, epidermis, and in tissues where cells undergo rapid proliferation and renewal and its expression is shown to be higher in proliferating cells. Overexpression of ING3 has been shown to inhibit cell growth and induce apoptosis. ING3 is reported to be down-regulated in squamous cell carcinoma of the head and neck.

Specificity

Clone 2A2 detected recombinant human ING3, but not INGs 1, 2, 4 or 5. Target band detection by Western blotting and immunocytochemistry. Staining by clone 2A2 was abolished using ING3 shRNA-transfected cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Immunogen

GST-tagged recombinant full-length human ING3.

Application

Detect ING3 using this mouse monoclonal Anti-ING3 Antibody, clone 2A2, Cat. No. MABC1185, validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected ING3 immunoreactivity in human small intestine tissue section.

Immunocytochemistry Analysis: A representative lot detected upregulated and downregulated ING3 immunoreactivity in 4% paraformaldehyde-fixed, 0.1% Triton X-100-permeabilized HEK293 cells transfected with ING3 and ING3 shRNA, respectively. Preabsorption with fixed ING3-overexpressing cells prevented subsequence immunocytochemical staining by clone 2A2 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Immunohistochemistry Analysis: A representative lot detected differential ING3 immunoreactivity among formalin-fixed paraffin-embedded human tissue sections, inluding hematopoietic tissues (bone marrow, lymphatic, thymus, and spleen) and epithelium of various tissue types (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Immunoprecipitation Analysis: A representative lot immunoprecipitated HA-tagged ING3 exogenously expressed in HEK293 cells (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Western Blotting Analysis: A representative lot detected endogenous ING3 in human HEK293 cells, a panel of murine tissues and cells (BALB-3T3 and NIH-3T3) as well as exogenously overexpressed ING3 in HEK293 cells, ING3 shRNA transfection abolished target band detection. Clone 2A2 detected bacterially rexpressed recombinant human ING3, but not INGs 1, 2, 4 or 5 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Western Blotting Analysis: A representative lot detected growth factor-induced ING3 expression in serum-starved HME human mammary fibroblasts immortalized with hTERT and RWPE-1 prostate epithelial cells immortalized with HPV18 (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Quality

Evaluated by Immunohistochemistry in human ovarian cancer tissue.



Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected ING3 immunoreactivity in human ovarian cancer tissue section.

Target description

46.74 kDa calculated. ~50 kDa reported (Nabbi, A., et al. (2015). Eur. J. Cell Biol. 94(5):214-222).

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.